Leptin Mouse Recombinant ( Leptin Mouse )

Catalog # PT_41391

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$201.00
DescriptionLeptin Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16.2 kDa.The Leptin is purified by proprietary chromatographic
Product Size: 200ug
Quantity

Data sheet

Formulation
The mouse Leptin was lyophilized from a concentrated (1mg/ml) solution containing 0.1% TFA.
Solubility
It is recommended to reconstitute the lyophilized Leptin in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Purity
Greater than 95.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE.
Description
Leptin Mouse Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 147 amino acids and having a molecular mass of 16.2 kDa.The Leptin is purified by proprietary chromatographic techniques.
Protein Background
A 16-kDa peptide hormone secreted from white adipocytes and implicated in the regulation of food intake and energy balance. Leptin provides the key afferent signal from fat cells in the feedback system that controls body fat stores.
Expression host
Escherichia Coli.
Synonyms
OB Protein, Obesity Protein, OBS, Obesity factor.
Reagent Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Stability
Lyophilized Leptin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Leptin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add
Amino acid sequence
MVPIQKVQDD TKTLIKTIVT RINDISHTQS VSAKQRVTGL DFIPGLHPIL SLSKMDQTLA VYQQVLTSLP SQNVLQIAND LENLRDLLHL LAFSKSCSLP QTSGLQKPES LDGVLEASLY STEVVALSRL QGSLQDILQQ LDVSPEC.
Biological Activity
Biological activity of Mouse Leptin is performed in two different mouse obesity models, ob/ob and NZO. Both strains of mice were treated via intraperitoneal injection once daily at a dose of 5ug Leptin/gram body weight for a period of 14 days. Significant
References
Title:Severe pulmonary metastasis in obese and diabetic mice.Publication: Article first published online: 22 SEP 2006 DOI:10.1002/ijc.22248 Copyright © 2006 Wiley-Liss, Inc.Link:http://onlinelibrary.wiley.com/doi/10.1002/ijc.22248/full
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