Leptin Antagonist Triple Mutant Ovine Recombinant, Pegylated ( Leptin tA Ovine, PEG )

Data sheet

Formulation

The protein was lyophilized from a concentrated (0.65mg/ml) solution with 0.003mM NaHCO3.

Solubility

It is recommended to reconstitute the lyophilized Leptin-Antagonist Triple Mutant Ovine Recombinant in sterile water or sterile 0.4% NaHCO3 adjusted to pH 8, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined by: (a) Gel filtration analysis. (b) Analysis by SDS-PAGE.

Description

Pegylated Leptin Antagonist Triple Mutant Ovine Recombinant is a single non-glycosilated polypeptide chain containing 146 amino and additional Ala at N-terminus acids and having a molecular mass of ~ 35.6 kDa, Leptin was mutated, resulting in L39A/D40A/F4

Expression host

Escherichia coli.

Reagent Appearance

White lyophilized (freeze-dried) powder.

Stability

Lyophilized Leptin Antagonist Triple Mutant Ovine Recombinant although stable at room temperature for several weeks, should be stored desiccated below -18C. Upon reconstitution at > 0.1 Lep-tA mutant mg/ml and up to 2mM and filter sterilization Leptin mut

Biological Activity

Pegylated triple antagonist is capable of inhibiting leptin-induced proliferation of BAF/3 cells stably transfected with the long form of human leptin receptor. Its in vitro activity is 6-8 fold lower than the non-pegylated antagonist but in vivo it has p