Oncostatin-M Human Recombinant, HEK ( OSM Human, HEK )

Data sheet

Formulation

The OSM was lyophilized from 1mg/ml in 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized OSM in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95% as obsereved by SDS-PAGE.

Inactivation

UV spectroscopy at 280 nm using the absorbency value of 0.85 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics).

Description

Oncostatin-M Human Recombinant produced in HEK cells is a glycosylated monomer, having a total molecular weight of 30kDa.The OSM is purified by proprietary chromatographic techniques.

Protein Background

Oncostatin M is a member of a cytokine family that includes leukemia-inhibitory factor, granulocyte colony-stimulating factor, and interleukin 6. This gene encodes a growth regulator which inhibits the proliferation of a number of tumor cell lines. It reg

Expression host

HEK.

Synonyms

OSM, MGC20461.

Stability

Lyophilized OSM although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution OSM should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carr

Physical Appearence

Sterile Filtered White lyophilized (freeze-dried) powder.

Biological Activity

The specific activity was determined by the dose-dependent stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) and is typically 2-10ng/ml.