Platelet-Derived Growth Factor BB Mouse Recombinant ( PDGF BB Mouse )

Catalog # PT_41715

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$201.00
DescriptionPlatelet-Derived Growth Factor BB Mouse Recombinant is a homodimeric, non-glycosylated, polypeptide chain containing 109 amino acids and having a molecular mass of 25 kDa (12.5 kDa per subunit).PDGF-BB is purified by proprietary
Product Size: 2ug
Quantity

Data sheet

Formulation
The protein was lyophilized from 10mM NaCitrate pH-3.0.
Solubility
It is recommended to reconstitute the lyophilized Platelet-derived Growth Factor-BB in sterile 100mM acetic acid and 0.1% BSA not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Purity
Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Description
Platelet-Derived Growth Factor BB Mouse Recombinant produced in E.coli, is a homodimeric, non-glycosylated, polypeptide chain containing 2x109 (total of 2 chains 218aa) amino acids and having a total molecular weight of 24.6 kDa.PDGF-BB is purified by pro
Protein Background
PDGF-BB is a member of the platelet-derived growth factor family. The four members of this family are mitogenic factors for cells of mesenchymal origin and are characterized by a motif of eight cysteines. This gene product can exist either as a homodimer
Expression host
Escherichia Coli.
Synonyms
Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, SIS, SSV, PDGF2, c-sis, FLJ12858, PDGF-BB, PDGF B-chain, Platelet-derived growth factor beta polypeptide, Becaplermin.
Reagent Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Stability
Lyophilized Platelet-derived Growth Factor BB although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF BB should be stored at 4°C between 2-7 days and for future use below -18°C. For long term stor
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Met-Ser-Leu-Gly-Ser.
Biological Activity
The biological activity is determined by the dose-dependant stimulation of the proliferation of human umbelical vein endothelial cells (HUVEC) using a concentration range of less than 1.6ng/ml.
References
1.Title: Analysis of fast protein phosphorylation kinetics in single cells on a microfluidic chipPublications: Lab on a Chip (2015)Link: http://pubs.rsc.org/en/content/articlehtml/2014/lc/c4lc00797b2. Title: The Mouse Cornea as a Transplantation Site for
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