Platelet Derived Growth Factor-AA Human Recombinant, HEK ( PDGF AA Human, HEK )

Data sheet

Formulation

The PDGF-AA was lyophilized from 1mg/ml in 1xPBS.

Solubility

It is recommended to reconstitute the lyophilized PDGF-AA in sterile water not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95% as obsereved by SDS-PAGE.

Description

PDGF-AA Human Recombinant produced in HEK cells is a glycosylated homodimer, having a molecular weight range of 35-45kDa due to glycosylation.The PDGF-AA is purified by proprietary chromatographic techniques.

Protein Background

PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGF is stored in platelet alpha-granules and released upon platelet

Expression host

HEK.

Synonyms

Glioma-derived growth factor, GDGF, Osteosarcoma-derived Growth Factor, ODGF, PDGF-AA, PDGF-1.

Stability

Lyophilized PDGF-AA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AA should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to ad

Physical Appearence

Sterile Filtered White lyophilized (freeze-dried) powder.

Biological Activity

The specific activity was determined by the dose-dependent stimulation of the proliferation of 3T3 cells and is typically 200-1000ng/ml.