GMCSF/ IL-3 Fusion Protein Human Recombinant ( PIXY321 Human )

Data sheet

Formulation

The protein was lyophilized after extensive dialysis against 10mM PBS, pH= 7.40.1 and 0.1% BSA.

Solubility

It is recommended to reconstitute the lyophilized PIXY321 in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined by1. Analysis by RP-HPLC.2. Analysis by SDS-PAGE.

Description

GMCSF/IL3 fusion protein Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain having a Mw of 30kDa.PIXY321 is purified by proprietary chromatographic techniques.

Protein Background

Synergism of IL3 together with GMCSF causes a rapid rise in neutrophils due to GMCSF and a slower rise in platelets due to IL3 which has strong stimulated effect on hematopoietic cells.The analog of GMCSF/IL3 is PIXY321. Results indicate that PIXY321 can

Expression host

Escherichia Coli.

Synonyms

PIXY321, GMCSF/ IL3.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized PIXY321although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PIXY321should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Biological Activity

The ED50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells (human erythroleukemic indicator cell line) is