Growth Hormone Placental 20kDa Human Recombinant ( pGH 20kDa Human )

Catalog # PT_41782

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$201.00
DescriptionGrowth Hormone Placental 20kDa Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 177 amino acids and having a molecular mass of 20498 Dalton. Predicted pI=8.20. Growth Hormone 20K placental is
Product Size: 10ug
Quantity

Data sheet

Formulation
pGH 20kDa was lyophilized from a concentrated (1mg/ml) solution with0.0045mM NaHCO3 previously adjusted pH 11.
Solubility
It is recommended to reconstitute the lyophilized Placental Growth Hormone in 0.4% NaHCO3or water adjusted to pH 11, not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Purity
Greater than 98.0% as determined by:(a) Analysis by SEC-HPLC.(b) Analysis by SDS-PAGE.
Description
Growth Hormone Placental 20kDa Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 177 amino acids and having a molecular mass of 20498 Dalton. Predicted pI=8.20. Growth Hormone 20K placental is devoid of lacto
Protein Background
GH is a member of the somatotropin/prolactin family of hormones which play an important role in growth control. The gene, along with four other related genes, is located at the growth hormone locus on chromosome 17 where they are interspersed in the same
Expression host
Escherichia Coli.
Synonyms
GHL, GHV, GH-V, hGH-V, PGH.
Reagent Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Stability
Lyophilized Growth Hormone 20K Placental although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution and filter sterilization GH 20K pl can be stored at 4°C for up to 4 weeks. For long term storage and mor
Amino acid sequence
The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Phe-Pro-Thr-Ile.
Isomerase Activity
0.001 650nm/ min-2. By measuring the turbidity increase at 650 nm due to insulin reduction (Holmgren, A. (1979) J. Biol. Chem. 254, 96279632). The activity is expressed as the ratio of the slope of a linear part of the turbidity curve to the lag time (Mar
Activity assay
0.5 umol active RNase A min-1 umol PDI-1. According to the re-activation of reduced and denatured RNase A (Lyles, M. M. and Gilbert, H. F. (1991) Biochemistry 30, 613-619).
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