Placenta Growth Factor-2 Human Recombinant ( PLGF 2 Human )

Data sheet

Formulation

Lyophilized from a concentrated (1mg/ml) solution containing BSA.

Solubility

It is recommended to reconstitute the lyophilized Placenta Growth Factor 2 in sterile 20mM acetic acid not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 80.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Placenta Growth Factor-2 Human Recombinant produced in insect cells is a homodimer, glycosylated polypeptide chain containing 2 x 152 amino acids and having a total molecular mass of 44 kDa. The PLGF-2 is purified by proprietary chromatographic techniques

Protein Background

PLGF is a growth factor active in angiogenesis, and endothelial cell growth, stimulating their proliferation and migration. It binds to receptor vegfr-1/flt1.PLGF-2 binds neuropilin-1 and 2 in a heparin-dependent manner.

Expression host

Insect Cells.

Synonyms

PIGF, PGF, PlGF-2, PLGF-2.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Placenta Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PLGF2 should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cyc

Biological Activity

PlGF-2 human Recombinant can bind to immobilized rh-sFlt-1 (100ng/well) with a linear range at 0.310ng/ml.

Quality Control Test

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMENS DDYDLMYVNL DNEIDNGLHP TEDPTPCDCG QEHSEWDKLF IMLENSQMRE RMLLQATDDV LRGELQRLRE ELGRLAESLA RPCAPGAPAE ARLTSALDEL LQATRDAGRR LARMEGAEAQ RPEEAGRALA AVLEELRQTR ADLHAVQGWA ARSWLPAGCE TAILFPMRSK KIFGSVHPVR PMRLESFSAC IW