Prolactin Antagonist Ovine Recombinant, Mutant ( Prolactin Ovine Antagonist, Mutant )

Data sheet

Formulation

Ovine Prolactin was lyophilized from a concentrated (1mg/ml) solution with 0.02%-0.03% NaHCO3.

Solubility

It is recommended to reconstitute the lyophilized Ovine Prolactin Antagonist in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 99.0% as determined by Gel Filtration & SDS-PAGE.

Description

Prolactin Ovine Antagonist Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 199 amino acids and an additional Ala at N-terminus and having a molecular mass of 23kDa. The mutant R129G is DES 9 amino acids truncated

Protein Background

Prolactin is a lactogenic hormone secreted by the adenohypophysis .Besides its major action on lactation, in some species prolactin exerts effects on reproduction, maternal behavior, fat metabolism, immunomodulation and osmoregulation.Prolactin has been s

Expression host

Escherichia Coli.

Synonyms

Mammotropin, Luteotropic hormone, Luteotropin, PRL, Prolactin.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Ovine Prolactin Antagonist although stable at room temperature for 3 weeks, should be stored desiccated below -18C. Upon reconstitution Ovine Prolactin Antagonist should be stored at 4C between 2-7 days and for future use below -18C.For long

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Thr-Pro-Val-Cys-Pro.

Biological Activity

Ovine Prolactin Antagonist mutant form is devoid of agonistic activity and capable of inhibiting biological activity of oPRL or other lactogenic hormones as evidenced by proliferation assay of Nb2 or other cells. The truncated form is more potent inhibito