Stem Cell Factor Human Recombinant, Sf9 ( SCF Human, Sf9 )

Data sheet

Formulation

The protein is supplied in 1xPBS, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized Stem Cell Factor in 10mM acetic acid not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 98.0% as determined by analysis by SDS-PAGE.

Description

Stem Cell Factor Human Recombinant produced in insect cells is a single, glycosylated polypeptide chain containing 165 amino acids and having a molecular mass of 18409 Dalton. The SCF is fused to a C-terminal His-tag (6xHis) and purified by proprietary ch

Protein Background

Stem cell factor / KIT ligand (SCF) is a cytokine which binds CD117(c-Kit). SCF is also known as "steel factor" or "c-kit ligand". SCF exists in two forms, cell surface bound SCF and soluble (or free) SCF. Soluble SCF is produced by the cleavage of surfac

Expression host

Sf9, Insect Cells.

Synonyms

Kit ligand Precursor, C-kit ligand, SCF, Mast cell growth factor, MGF, SF, KL-1, Kitl, DKFZp686F2250.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized KIT ligand although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SCF should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to ad

Biological Activity

The ED50 as determined by the dose-dependent stimulation of Human TF-1 cells is typically 1-5 ng/ml.