Vascular Endothelial Growth Factor Related Protein Human Recombinant ( VEGF C Human )

Data sheet

Formulation

Each mg of VEGF-C Human contains 50mg BSA and 1xPBS as buffer.

Solubility

It is recommended to reconstitute the lyophilized VEGF-C in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 90.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

VEGF-C Human Recombinant- contains 129 amino acids residues and was fused to a His- tag (6x His) at the C-terminal end. As a result of glycosylation VEGF-C migrates as an 18-24 kDa protein in SDS-PAGE under reducing conditions.

Protein Background

VEGF-C, also known as Vascular Endothelial Growth Factor Related Protein (VRP), is a recently discovered VEGF growth factor family member that is most closely related to VEGF-D. Human VEGF-C cDNA encodes a pre-pro-protein of 416 amino acids residues. It i

Expression host

Sf9, Insect Cells.

Synonyms

VEGF-C, Vascular endothelial growth factor C, VRP, Flt4 ligand, Flt4-L, Vascular endothelial growth factor-related protein, VEGFC.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized VEGF-C although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF-C should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles.

Biological Activity

Measured by its ability to bind to the VEGFR-3/FLT-4 receptor in the Ba/F3-hVEGFR-3 assay. The ED50 in this assay is about 4ng/ml corresponding to a Specific Activity of 250,000IU/mg.