Vascular Endothelial Growth Factor-B Human Recombinant ( VEGFB Human )

Data sheet

Formulation

The VEGFB protein solution contains 20mM Tris-HCl buffer pH 7.5 & 10% glycerol.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

VEGFB Human Recombinant produced in E.Coli is a double, non-glycosylated, polypeptide chain containing 207 amino acids (22-207) and having a molecular mass of 21.6 kDa (molecular weight on SDS-PAGE will appear higher).The VEGFB is fused to a 20 amino acid

Protein Background

VEGFB is a growth factor for endothelial cells. VEGFB 167 a.a. binds heparin and neuropilin-1 while the binding to neuropilin-1 of VEGFB 186 a.a. is regulated by proteolysis. Overexpression of VEGFB in mouse heart alters cardiac lipid metabolism and induc

Expression host

Escherichia Coli.

Synonyms

Vascular endothelial growth factor B, VEGF-B, VRF, VEGFL, VEGF-related factor, VEGFB.

Reagent Appearance

Sterile Filtered colorless liquid formulation.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MPVSQPDAPG HQRKVVSWID VYTRATCQPR EVVVPLTVEL MGTVAKQLVP SCVTVQRCGG CCPDDGLECV PTGQHQVRMQ ILMIRYPSSQ LGEMSLEEHS QCECRPKKKD SAVKPDRAAT PHHRPQPRSV PGWDSAPGAP SPADITHPTP APGPSAHAAP STTSALTPGP AAAAADAAAS SVAKGGA.

Biological Activity

Measured in a cell proliferation assay using NIH-3T3 mouse embryonic fibroblast. The ED50 for this effect is