Vascular Endothelial Growth Factor Human Recombinant, CHO ( VEGF Human, CHO )

Data sheet

Formulation

The protein was lyophilized from a Phosphate- Buffered Saline, pH 7.4.

Solubility

It is recommended to reconstitute the lyophilized Vascular Endothelial Growth Factor Human in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 97.0% as determined by SDS-PAGE.

Inactivation

Protein quantitation was carried out by two independent methods1. UV spectroscopy at 280 nm using the absorbency value of 0.75 as the extinction coefficient for a 0.1% (1mg/ml) solution at pH 8.0 This value is calculated by the PC GENE computer analysis p

Description

Vascular Endothelial Growth Factor Human Recombinant produced in CHO cells is a double, glycosylated, polypeptide chain containing 165 amino acids and migrates as 44 kDa in SDS-PAGE under non-reducing conditions. The VEGF is purified by proprietary chroma

Protein Background

Vascular endothelial growth factor is an important signaling protein involved in both vasculogenesis and angiogenesis. As its name implies, VEGF activity has been mostly studied on cells of the vascular endothelium, although it does have effects on a numb

Expression host

Chinese Hamster Ovarian Cell.

Synonyms

Vascular endothelial growth factor A, VEGF-A, Vascular permeability factor, VPF, VEGF, MGC70609.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Vascular Endothelial Growth Factor Human although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution VEGF Human Recombinant should be stored at 4°C between 2-7 days and for future use below -18

Biological Activity

The protein was tested in HUVEC cells, the ED50 for this effect was found to be 2-6ng/ml.