Exendin-4 Recombinant ( Exendin 4 )

Data sheet

Formulation

The Exendin-4 peptide was lyophilized after extensive dialyses against 20mM PBS and contains 4% mannitol.

Solubility

It is recommended to reconstitute the lyophilized Exendin Recombinant in sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Exendin-4 Recombinant is a novel 39-amino acids peptide having a molecular mass of 4186.7 Dalton, which shares 53%, sequence homology with GLP-17-36 amide and interacts with the same membrane receptor. The Exendin-4 is purified by proprietary chromatograp

Protein Background

Exedin-4 Recombinant is a glucagon like peptide-1 receptor agonist. The native hormone is produced in the gut of Gila monster Heloderma suspectrum (a type of reptile found in the desert) that stimulates insulin production without causing threateningly low

Expression host

Escherichia Coli.

Synonyms

Exenatide.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Exendin Recombinant although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Exendin-4 should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is re

Amino acid sequence

The sequence determined and was found to be His-Gly-Glu-Gly-Thr-Phe-Thr-Ser-Asp-Leu-Ser-Lys-Gln-Met-Glu-Glu-Glu-Ala-Val-Arg-Leu-Phe-Ile-Glu-Trp-Leu-Lys-Asn-Gly-Gly-Pro-Ser-Ser-Gly-Ala-Pro-Pro-Pro-Ser.

Biological Activity

1. Regulates Glucose levels rapidly2. Reduces Insulin resistence 3. Reduces Glucagon4. Reduces HbA1c5. Stimulates beta cell growth which stimulates insulin production.