Glucagon Human Recombinant ( Glucagon Human )

Data sheet

Formulation

Each mg of recombinant Glucagon was formulated with 100mg of lactose.

Solubility

It is recommended to reconstitute the lyophilized Glucagon sterile 18M-cm H2O not less than 100 ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Glucagon Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 29 amino acids and having a molecular mass of 3483 Dalton. The Glucagon is purified by proprietary chromatographic techniques.

Protein Background

Glucagon is an important hormone involved in carbohydrate metabolism. The hormone is synthesized and secreted from alpha cells (a-cells) of the islets of Langerhans, which are located in the endocrine portion of the pancreas. Glucagon is released when the

Expression host

Escherichia Coli.

Synonyms

GLP1, GLP2, GRPP.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Glucagon although stable at room temperature for 3 weeks, should be stored at 40C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).

Amino acid sequence

The sequence of the first five N-terminal amino acids was determined and was found to be His-Ser-Gln-Gly-Thr.

Biological Activity

The activity is determind by comparing, under certain conditions, the hyperglycemic effect it produces with that produced by the international standard or by reference preparation calibrated in IU and is found to be 1 IU/mg.

Product Description

Immunoreactive with sera HAV-infected individuals.