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Fumonisin Food ELISA Kit
NR-R10194
Fumonisin Food ELISA KitÂ
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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC OR THERAPEUTIC PURPOSES
Data sheet
| Category | Food ELISA kits |
| Background | Fumonisin B1 and B2 are mycotoxins, which are produced by Fusarium moniliforme, F. solani and other types of fusarium. These toxins show an extreme stability against high temperatures (up to 100 C), and they can remain active in contaminated food for years. Fumonisin can be found in maize, oats and other types of grain. Worldwide a contamination in maize of 60% has been detected. When ingested by animals, fumonisin leads to neurotoxicity, hepatotoxicity and lung edema, mainly in horses and pigs. Therapeutic measures are the change of the grain given to the animals, or the administration of diuretic drugs. In human patients, hints for the appearance of esophagus cancer could be associated with the exposition to fumonisin B1. Values assessed for the acute toxicity are 8 mg per kg weight and for the chronic situation 25 mg/kg in feed stuff. These are relatively high concentrations, but according to the Good Manufacturing Practice, the content of fumonisin B1 in food intended for humans should not exceed 1 mg/kg. The usual methods for detection of fumonisin in maize are liquid and thin-layer chromatography. The Fumonisin Food ELISA test kit is very sensitive and allows the detection of trace amounts. |
| Assay principle | The Fumonisin Food ELISA Kit is a quantitative test based on the principle of the enzyme linked immunosorbent assay. A microtiter plate is coated with antibodies raised against mouse immunglobulins. The standards and samples respectively are pipetted together with a fumonisin- peroxidase conjugate and a mouse-anti-fumonisin antibody into the appropriate wells of the Fumonisin ELISA Kit. The conjugate competes with the fumonisin of samples/standards for the limited number of antibody sites. Simultaneously the anti-fumonisin antibody is bound to the anti-mouse antibody coated on the microtiter plate of the Fumonism ELISA Kit. |
| Assay Type | Competitive ELISA |
| Kit Type | Colorimetric |
| Assay Time | about 80 min |
| Sensitivity | 0.5 ng/mL |
More info
Materials Provided with Fumonisin Food ELISA Kit:
1. Microtiter plate: 12 strips with 8 breakable wells coated with anti-mouse immunoglobulin
2. Fumonisin Standards (0; 0.5; 2; 5; 10; 25 ng/mL): 6 vials with 1.0 mL
3. Conjugate (Fumonisin-Peroxidase): 6 mL
4. Anti-Fumonisin Antibody, 6 mL
5. Substrate Solution (TMB): 15 mL
6. Stop Solution (0.5 M H2SO4): 15 mL
7. Sample diluent (PBS) 2x
8. Washing Solution (PBS + Tween 20): 60 mL as 10x
9. Two plastic foils to cover the strips during the incubation
10. Plastic bag to store unused microtiter strips
11. Instruction Manual
Materials required but not provided:
1. Freshly distilled or deionized water
2. Dispensing system and/or pipette
3. EIA kit Microplate washer
4. EIA kit Microplate Reader with 450nm wavelength