Procalcitonin Human Recombinant ( Procalcitonin Human )

Data sheet

Formulation

Lyophilized from 10mM sodium phosphate pH-7.5.

Solubility

It is recommended to reconstitute the lyophilized procalcitonin sterile 18M-cm H2O at 100 ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 95.0% as determined by SDS-PAGE.

Description

Procalcitonin Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 116 amino acids and having a molecular mass of 12.8 kDa.The Procalcitonin is purified by standard chromatographic techniques.

Protein Background

Procalcitonin is a peptide hormone mainly produced by the C cells of the thyroid and certain endocrine cells of the lung. Under normal expression conditions, procalcitonin is immediately cleaved into three specific fragments, an N terminal residue, calcit

Expression host

Escherichia Coli.

Synonyms

Procalcitonin, PCT.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized procalcitonin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution procalcitonin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recom

Amino acid sequence

APFRSALESS PADPATLSED EARLLLAALV QDYVQMKASE LEQEQEREGS SLDSPRSKRC GNLSTCMLGT YTQDFNKFHT FPQTAIGVGA PGKKRDMSSD LERDHRPHVS MPQNAN.

Biological Activity

Human Procalcitonin was tested using Biomerieux reagents on the Min-Vidas. The assay was run at 80ng/ml with a typical recovery of 80 percent.

References

Title:A sandwich assay for procalcitonin detection in POCT applications.Publication:Advanced Biomedical and Clinical Diagnostic Systems VII, edited by Anita Mahadevan-Jansen,Tuan Vo-Dinh, Warren S. Grundfest, Proc. of SPIE Vol. 7169, 716905© 2009 SPI