Disulfide-Bond Isomerase Recombinant ( DsbC )

Data sheet

Formulation

1 mg/ml solution containing 20mM phosphate buffer pH 7.5 and 20mM EDTA.

Purity

Greater than 95.0% as determined by(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

Disulfide-Bond Isomerase Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 216 amino acids (21-236) and having a molecular mass of 23.5 kDa.DsbC is purified by proprietary chromatographic techniques.

Protein Background

Dsb proteins (DsbA, DsbB, DsbC, and DsbD) catalyze formation and isomerization of protein disulfide bonds in the periplasm of Escherichia coli. DsbC is periplasmic enzyme known as a disulfide isomerase and can convert aberrant disulfide bonds to correct o

Expression host

Escherichia Coli.

Synonyms

DsbC, Thiol:disulfide interchange protein dsbC.

Reagent Appearance

Sterile filtered colorless solution.

Stability

Disulfide-Bond Isomerase Recombinant although stable at 4°C for 1 week, should be stored desiccated below -18°C.Please prevent freeze-thaw cycles.

Amino acid sequence

DDAAIQQTLA KMGIKSSDIQ PAPVAGMKTV LTNSGVLYIT DDGKHIIQGP MYDVSGTAPVNVTNKMLLKQ LNALEKEMIV YKAPQEKHVI VFTDITCGY CHKLHEQMAD YNALGITVRYLAFPRQGLDS DAEKEMKAIW CAKDKNKAFD DVMAGKSVAP ASCDVDIADH YVLGVQLGVSGTPAVVLSNG TLVPGYQPPK EMKEFLDEHQ KMTSGK.