Hepatitis C Virus Core Genotype-3b Recombinant ( HCV Core Genotype-3b )

Data sheet

Storage

HCV Core Genotype-3b although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.

Formulation

25mM Tris-Hcl pH 8, 0.2% Triton-X, 1.5M urea and 50% glycerol.

Purity

HCV Core Genotype-3b protein is >95% pure as determined by 10% PAGE (coomassie staining).

Applications

HCV Core Genotype-3b antigen is suitable for ELISA and Western blots, excellent antigen for detection of HCV with minimal specificity problems.

Description

The E.coli derived recombinant protein contains the HCV core nucleocapsid immunodominant regions, amino acids 2-119. The protein is fused to a GST Tag at N-terminus.

Protein Background

HCV is a small 50nm, enveloped, single-stranded, positive sense RNAvirus in the family Flaviviridae. HCV has a high rate of replication with approximately one trillion particles produced each day in an infected individual. Due to lack of proofreading by t

Purification Method

HCV Core Genotype-3b protein was purified by proprietary chromatographic technique.

Introdcution

Recombinant Albumin can be used as a media culture supplement at concentrations up to 5 grams per liter. Gradual adaptation of cell lines over several passages to a concentration of 0.5gr to 2gr per liter.