Polymerase ( DNA directed ), Lambda Human Recombinant ( POLL Human )

Data sheet

Formulation

The POLL solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 30% glycerol.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

POLL Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 320 amino acids (1-300) and having a molecular mass of 36.0 kDa.POLL is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatog

Protein Background

DNA polymerase POLL is involved in base excision repair (BER). POLL is a low-fidelity polymerase that takes part in both spontaneous and DNA damage-induced mutagenesis. Furthermore, POLL takes part in the mutagenic bypass of T-T dimers. Pol lambda possess

Expression host

E.coli.

Synonyms

Polymerase (DNA directed) lambda, Pol beta2, DNA polymerase beta-2, DNA polymerase kappa, BETAN, POLKAPPA, DNA polymerase beta-N, EC 2.7.7.7, EC 4.2.99.

Reagent Appearance

Sterile Filtered colorless solution.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MLMHHQKYLQ RFLGGKREKK QKEACSIPGI GKRMAEKIIE ILESGHLRKL DHISESVPVL ELFSNIWGAG TKTAQMWYQQ GFRSLEDIRS QASLTTQQAI GLKHYSDFLE RMPREEATEI EQTVQKAAQA FNSGLLCVAC GSYRRGKATC GDVDVLITHP DGRSHRGIFS RLLDSLRQEG FLTDDLVSQE ENGQQQKYLG VCRLPGPGRR HR