Threonyl-tRNA Synthetase Human Recombinant ( PL 7 Human )

Data sheet

Formulation

PL-7 is supplied in 20mM HEPES buffer pH-8, 200mM sodium chloride, and 20% glycerol.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

Threonyl-tRNA Synthetase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 83,312 Dalton. PL-7 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.

Protein Background

Threonyl-tRNA Synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 83 kDa protein is an autoantigen recognized by PL-7 antibodies which occur

Expression host

Sf9 insect cells.

Synonyms

Threonyl-tRNA synthetase cytoplasmic, EC 6.1.1.3, Threonine-tRNA ligase, ThrRS, MGC9344, PL-7, TARS.

Reagent Appearance

Sterile Filtered clear solution.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.

Immunological functions

1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test).

Coating concentration

0.3-0.8 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination.