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Threonyl-tRNA Synthetase Human Recombinant ( PL 7 Human )
DescriptionThreonyl-tRNA Synthetase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 83,312 Dalton. PL-7 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques.SourceSf9
Data sheet
| Formulation | PL-7 is supplied in 20mM HEPES buffer pH-8, 200mM sodium chloride, and 20% glycerol. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Description | Threonyl-tRNA Synthetase Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 83,312 Dalton. PL-7 is expressed with a -6xHis tag and purified by proprietary chromatographic techniques. |
| Protein Background | Threonyl-tRNA Synthetase is a member of the aminoacyl-tRNA synthetase family, key enzymes of protein biosynthesis which charge tRNA molecules with the respective amino acids. This 83 kDa protein is an autoantigen recognized by PL-7 antibodies which occur in a subset of patients with polymyositis and dermatomyositis. Preliminary data suggest that PL-7 antibodies (similar to Jo-1 antibodies) indicate an increased risk for lung involvement, but this needs to be confirmed for a larger number of cases. |
| Expression host | Sf9 insect cells. |
| Synonyms | Threonyl-tRNA synthetase cytoplasmic, EC 6.1.1.3, Threonine-tRNA ligase, ThrRS, MGC9344, PL-7, TARS. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Storage Conditions | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Immunological functions | 1. Binds IgG-type human auto-antibodies.2. Standard ELISA test (checker-board analysis of positive/negative sera panels immuno-dot test). |
| Coating concentration | 0.3-0.8 ug/ml (depending on the type of ELISA plate and coating buffer).Suitable for biotinylation and iodination. |