Three Prime Repair Exonuclease 2 Human Recombinant ( TREX2 Human )

Data sheet

Storage

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Formulation

The TREX2 protein solution (1mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 200mM NaCl, 5mM DTT and 30% glycerol.

Purity

Greater than 95% as determined by SDS-PAGE.

Description

TREX2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 256 amino acids (1-236a.a.) and having a molecular mass of 28.0 kDa. TREX2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic tech

Protein Background

TREX2 holds a 3-prime-to-5-prime exonuclease activity and eliminates mismatched, modified, fragmented, and normal nucleotides to produce the appropriate 3-prime termini for following steps in the DNA metabolic pathways. TREX2 has a role in DNA replication

Expression host

Escherichia Coli.

Synonyms

Three Prime Repair Exonuclease 2, 3'-5' exonuclease TREX2 long form.

Reagent Appearance

Sterile Filtered clear solution.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MSEAPRAETF VFLDLEATGL PSVEPEIAEL SLFAVHRSSL ENPEHDESGA LVLPRVLDKL TLCMCPERPF TAKASEITGL SSEGLARCRK AGFDGAVVRT LQAFLSRQAG PICLVAHNGF DYDFPLLCAE LRRLGARLPR DTVCLDTLPA LRGLDRAHSH GTRARGRQGY SLGSLFHRYF RAEPSAAHSA EGDVHTLLLI FLHRAAELLA WA