Urease Recombinant ( Urease )

Catalog # PT_42359

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$201.00
DescriptionUrease (EC 3.5.1.5) is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The reaction occurs as follows:(NH2)2CO + H2O = CO2 + 2NH3.In 1926 James Sumner showed that urease is a protein. Urease is found in
Product Size: 1mg
Quantity

Data sheet

Formulation
Each mg of protein contains 420ug Potassium Phosphate and 30ug EDTA Na2.
Solubility
It is recommended to reconstitute the lyophilized Urease in sterile 18M-cm H2O.
Purity
Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Description
Urease (EC 3.5.1.5) is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The reaction occurs as follows:(NH2)2CO + H2O = CO2 + 2NH3.In 1926 James Sumner showed that urease is a protein. Urease is found in bacteria, yeast and
Expression host
Escherichia Coli.
Reagent Appearance
Sterile Lyophilized Powder.
Stability
Urease although stable at 4°C for 3 weeks, should be stored desiccated below -18°C. Please prevent freeze-thaw cycles.
Unit Definition
One Unit oxidizes one micromole of NADH per minute at 25°C, at pH 7.6.
Biological Activity
The activity was found to be 141U/mg powder.
Activity assay
GRGGAAAPTA PNGTLEAELE RRWESLVALS LARLPVAAQP KEAAVQSGAG DYLLGIKRLR RLYCNVGIGF HLQALPDGRI GGAHADTRDS LLELSPVERG VVSIFGVASR FFVAMSSKGK LYGSPFFTDE CTFKEILLPN NYNAYESYKY PGMFIALSKN GKTKKGNRVS PTMKVTHFLP RL.
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