| Assay principle |
During the first incubation, Giardia and/or Cryptosporidium antigens present in stool specimens are
captured by antibodies attached to the microwells. The wells are incubated and washed before antiGiardia and anti-Cryptosporidium antibodies conjugated to peroxidase are added. The enzyme conjugate
will “sandwich” any antigen bound to the wells. After washings to remove any unbound enzymes, a
Chromogen is added, which develops a blue color in the presence of the enzyme complex. The Stop
Solution ends the reaction and turns the blue color to yellow. If no antigen is captured, or if there is an
insufficient level of antigen, no colored reaction will take place. |
