Data sheet
| Assay Range | 31.2 - 2000 pg/ml |
More info
Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse Car8 standard: 1 ng/vial - 2.
2. One 96-well plate precoated with anti-Mouse Car8 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Mouse Car8 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
There are 16 isoforms in CA gene family in most mammals, of which 13 isozymes (CA I, II, III, IV, VA, VB, VI, VII, IX, XII, XIII, XIV, and XV) are catalytically active. CARP VIII was originally identified based on specific expression patterns in mouse brain by selecting 35 cDNA clones. Expression of CARP VIII was reported in a wide variety of mouse tissues both at the mRNA and protein levels. CARP VIII has been expressed as glutathione-S-transferase fusion protein (GST-HCARP VIII). CARP VIII expression might inhibit caspase-3 activation. Car8 may be a novel oncofetal antigen, which plays a role in tumorigenesis of non-small cell lung carcinomas.
