More info
Assay range | 0.625-20 ng/ml |
Sensitivity | 0.6 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | plasma, serum, and cell culture supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse Transferrin standard: 1 vial
2. One 96-well plate coated with Mouse Transferrin Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Mouse Transferrin Ab (50x): 140 µL
5. Streptavidin-HRP (100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Transferrin is a plasma glycoprotein encoded by the TF gene in human. Transferrin binds iron very tightly and reversibly. It plays a key role in controlling the level of free iron in biological fluids. Transferrin contains two specific high-affinity Fe(III) binding sites. The affinity of transferrin for Fe(III) is extremely high (1023 M−1 at pH 7.4) but decreases progressively with decreasing pH below neutrality. Low transferrin level in plasma could associate with anemia and chronic liver disease, while high plasma level of transferrin could indicate iron deficiency anemia.