More info
Assay range | 0.391-400 ng/ml |
Sensitivity | 0.3 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | plasma, serum, urine, and cell culture supernatants |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Rat Plasminogen standard: 1 vial
2. One 96-well plate coated with Rat Plasminogen Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Rat Plasminogen Ab (70x): 105 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Plasminogen is a single chain glycoprotein zymogen that is primarily synthesized in the liver and circulated in plasma with a molecular weight of 90 kDa. Human Plasminogen contains an N-terminal activation peptide, five characteristically folded kringle domains, and a peptidase S1 domain. Cleavage of the activation peptide produces mature Plasminogen, which can be further cleaved between Arg580 and Val581 by tissue plasminogen activator (tPA) to produce the disulfide-linked two-subunit enzyme plasmin that plays a key role in breakdown of fibrin clots. The plasminogen system plays a role in macrophage recruitment, arterial stenosis, atherosclerosis, aneurysm formation, skin and corneal wound healing, glomerulonephritis, and neovascularization.