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Rat Haptoglobin Hpt ELISA Kit

AP-E100276

$570.00

More info

Assay range

937 - 60,000 ng/ml

Sensitivity

900 ng/ml

Specificity

No cross-reaction with other related substances detected

Size

96T

Storage

Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC

Assay Principle

Competitive ELISA

Sample Volume

25 µL final volume, dilution factor varies on samples

Sample Type

Urine and cell culture supernatants

Detection Method

Chromogenic

 

 

Kit Components

 

 1. Recombinant Rat  Hpt standard: 1 vial

 2. One 96-well plate coated with Rat  Hpt  Ab

 3. Diluent buffer (10x): 30 mL - 1

 4. Biotinylated Rat  Hpt:  1 vial

 5. Streptavidin-HRP(100x): 80 µL

 6. TMB developing agent: 8 mL x1

 7. Stop solution: 12 mL x1

 8. Washing solution (20x): 30 mL x2

 

 

Background

 

Haptoglobin is a 45-48 kDa acute phase glycoprotein belonging to the peptidase S1 family. The full-length human Haptoglobin precursor protein is a 388 amino acid (aa) single-chain polypeptide which can be proteolytically cleaved into disulfide-linked α- and β-chains then dimerizing through α-chain linkages to create a tetramer. Human Haptoglobin shares 80% aa identity with mouse Haptoglobin. Haptoglobin binds to hemoglobin with extremely high-affinity. It detoxifies free hemoglobin dimers in the circulation by burying the oxidative portions and chaperoning hemoglobin to the scavenger receptor CD163 on the surface of monocytes and macrophages for clearance. It is an important marker of hemolysis. High haptoglobin level in plasma may be an indicator of increased cardiovascular risk in obese men, inflammation, atherosclerosis, and systemic sclerosis.

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