Human Apolipoprotein A-II ELISA Kit

Catalog # AP-E100252

Print

$604.20
Quantity

Assay   range

3.9-4,000   ng/ml

Sensitivity

3.0 ng/ml  

Specificity

No   cross-reaction with other related substances detected

Size

96T

Storage

Store   at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC

Assay   Principle

Sandwich   ELISA

Sample   Volume

50 µL   final volume, dilution factor varies on samples

Sample   Type

Serum,   plasma, body fluids or cell lysates/supernatants

Detection   Method

Chromogenic

 

 

Kit Components

 

 1. Recombinant  Human ApoA2  standard: 1 vial

 2. One 96-well plate coated with Human ApoA2  Ab

 3. Diluent buffer (10x): 30 mL - 1

 4. Biotinylated Human ApoA2  Ab (50x): 140 µL

 5. Streptavidin-HRP(100x): 80 µL

 6. TMB developing agent: 8 mL x1

 7. Stop solution: 12 mL x1

 8. Washing solution (20x): 30 mL x2

 

 

Background

 

Apolipoprotein A-II (ApoA-II), also known as Apolipoprotein A2 (ApoA2), is the second most abundant apolipoproteins in human plasma HDL, comprising about 25% of the protein mass. Synthesized by the liver and intestine as a 100 amino acid (aa) precursor protein, ApoA-II is processed to 77 aa mature protein. In plasma, ApoA-II exists as a monomer, homodimer of 17.4 kDa, or heterodimer with ApoE and ApoD. Mature human ApoA2 shares 96% aa sequence identity with chimpanzee ApoA2 and 48% -66% aa sequence identity with bovine, equine, mouse, and rat ApoA2.

It has been reported that ApoA-II plays roles in HDL remodeling, cholesterol efflux, modulating HDL interaction with enzymes and receptors, triglyceride metabolism, and atherosclerosis. ApoA-II is inversely associated with risk of future coronary artery disease. In addition, serum levels of an isoform of ApoA-II have been identified as a potential marker for prostate cancer.

New