Data sheet
| Assay Range | 7.8 - 500 pg/ml |
More info
Sensitivity
5 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse TROY standard:5 ng/vial - 2.
2. One 96-well plate precoated with anti-mouse TROY Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-mouse TROY Ab: 1 vial, dilution 1:2000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
TNFRSF19 is one of the members of the TNFreceptorsuperfamily of proteins. It is a type I cell surface receptor protein containing the highly conserved TNFR cysteine-rich motifs in the extracellular domain and a TNF receptor-associated factor (TRAF)-binding sequence in the cytoplasmic domain. TNFRSF19 can mediate activation of c-Jun and NF-kappa-B and promote caspase-independent cell death. In neonatal mice, it is mainly expressed in hair follicles and neuron-like cells in the cerebellum, but not in the skin epidermis. TNFRSF19 can be detected in embryonic brain and epithelium, and at lower levels in adult heart, lung and liver. In addition, TNFRSF19 is the first TNFRSF member that is identified as a biomarker for melanoma.
