Human Complement Factor I ELISA Kit

Kit Components

 1. Recombinant  Human Factor I  standard: 1 vial

 2. One 96-well plate coated with  Human Factor I  Ab

 3. Diluent buffer (10x): 30 mL - 1

 4. Biotinylated Human Factor I: I vial

 5. Streptavidin-HRP(100x): 80 µL

 6. TMB developing agent: 8 mL x1

 7. Stop solution: 12 mL x1

 8. Washing solution (20x): 30 mL x2

Background

Complement Factor I, also known as C3b/C4b inactivator, is a glycosylated plasma serine proteinase of complement regulatory enzyme. It is synthesized as a 583 amino acid single-chain precursor (88 kDa) which is processed into a heterodimer consisting of disulfide-linked heavy (50 kDa) and light (38 kDa) chains. It circulates in an inactive zymogen-like state. In the presence of additional regulatory cofactors such as C4b-binding protein (C4BP), factor H, complement receptor 1, and membrane cofactor protein, Factor I can cleave and inactivate the complement components C3b and C4b to regulate the levels of C3 convertases. Factor I deficiency is associated with atypical hemolytic uraemic syndrome, primary glomerulonephritis, and recurrent pyogenic infections.