More info
Assay Range | 375-24,000 ng/ml |
Sensitivity | 375 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Competitive ELISA |
Sample Volume | 25 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human Factor I standard: 1 vial
2. One 96-well plate coated with Human Factor I Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human Factor I: I vial
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7. Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Complement Factor I, also known as C3b/C4b inactivator, is a glycosylated plasma serine proteinase of complement regulatory enzyme. It is synthesized as a 583 amino acid single-chain precursor (88 kDa) which is processed into a heterodimer consisting of disulfide-linked heavy (50 kDa) and light (38 kDa) chains. It circulates in an inactive zymogen-like state. In the presence of additional regulatory cofactors such as C4b-binding protein (C4BP), factor H, complement receptor 1, and membrane cofactor protein, Factor I can cleave and inactivate the complement components C3b and C4b to regulate the levels of C3 convertases. Factor I deficiency is associated with atypical hemolytic uraemic syndrome, primary glomerulonephritis, and recurrent pyogenic infections.