More info
Assay Range | 234.5-15,000 pg/mL |
Sensitivity | 230 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human C9 standard: 1 vial
2. One 96-well plate coated with Human C9 Ab
3. Diluent buffer (10x): 30 mL - 1
4. Biotinylated Human C9 Ab (50x): 140 µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7 . Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Human C9 is a 537 amino acid (aa) single-chain glycosylated (7.8%) protein with a molecular weight of 71KDa. C9 binds to the C5b-8 complex and forms the mature membrane attack complex (MAC) on cell membranes. The protease α-thrombin cleaves C9 at 294 aa residues from the carboxy-terminal end and produces two single-chain polypeptides: a hydrophilic C9a and a hydrophobic C9b. In the presence of membrane bound components C5b-8, C9 inserts into the phopholipid bilayer and becomes a pore-forming subunit of the membrane attack complex (MAC) on target membranes. C9 deficiency has been associated with a significantly increased risk of developing meningococcal meningitis.