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Data sheet
| Background | Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. In humans, TGF-β1 is encoded by the TGFB1 gene. TGF-β is a multifunctional set of peptides that controls proliferation, differentiation, and other functions in many cell types. TGF-β acts synergistically with TGFA in inducing transformation. It also acts as a negative autocrine growth factor. Dysregulation of TGF-β activation and signaling may result in apoptosis. Many cells synthesize TGF-β and almost all of them have specific receptors for this peptide. TGF-β1, TGF-β2, and TGF-β3 all function through the same receptor signaling systems. TGF-β1 was first identified in human platelets as a protein with a molecular mass of 25 kilodaltons with a potential role in wound healing. It was later characterized as a large protein precursor (containing 390 amino acids) that was proteolytically processed to produce a mature peptide of 112 amino acids.TGF-β1 plays an important role in controlling the immune system, and shows different activities on different types of cell, or cells at different developmental stages. Most immune cells (or leukocytes) secrete TGF-β1. |
| Assay principle | This Human TGF beta 1 ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. Human TGF beta 1 specific antibody has been precoated onto 96-well plate. The test samples and the biotinylated Human TGF beta 1 specific detection antibody are added to the wells subsequently and then followed by washing the plate. Streptavidin-HRP is added and unbound conjugates are washed away with Wash Buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic Stop Solution. The density of yellow is proportional to theHumanTGFbeta1amountofsamplecapturedinplate. |
| Alternate Names | DPD1; latency-associated peptide; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1 |
| Gene ID | 7040 |
| UniProt ID | P01137 |
More info
|
Assay Range |
15.6 - 1,000 pg/mL |
|
Sensitivity |
10.5 pg/mL |
|
Size |
96T |
|
Storage |
Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
|
Assay Principle |
Sandwich ELISA |
|
Sample Volume |
100 µL final volume, dilution factor varies on samples. Human serum dilution betweeen 1:40 to 1:100. |
|
Detection Method |
Colorimetric |
Kit Components
1. Recombinant Human TGF beta1 standard: 2 vials
2. One 96-well plate coated with anti- Human TGF beta1 Ab
3. Sample diluent buffer: 12 mL - 2
4. Detection antibody: 60 µL, dilution 1:180
5. Streptavidin-HRP: 300 µL, dilution 1:40
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. Chromogen-A, 6 mL; Chromogen-B, 6 mL.
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1.
| Precision | ||||||
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| CV (%) | 4.3 | 4.7 | 3.9 | 6.3 | 8.5 | 9.6 |
| Average CV (%) | 4.3 | 8.1 | ||||
| Recovery | ||||||
| Standard Added Value | 20- 800 pg/ml | |||||
| Recovery % | 93 - 105% | |||||
| Average Recovery % | 99% | |||||
| Linearity | ||||||
| Average Percentage of Expected Value (%) | ||||||
| Sample Dilution | Plasma | Serum | ||||
| 2 x | 102% | 96% | ||||
| 4 x | 105% | 99% | ||||
| 8 x | 103% | 98% | ||||
product citations
Susianti H, Gunawan A, Putri JF, Purnomo BB, Handono K, Kalim H
Bioinformation. 2014 Aug 30;10(8):487-90
Sample: Urine
2. Analysis of urinary TGF-β1, MCP-1, NGAL, and IL-17 as biomarkers for lupus nephritis
Susianti H, Iriane VM, Dharmanata S, Handono K, Widijanti A, Gunawan A, Kalim H
Pathophysiology. 2015 Mar;22(1):65-71
Sample: Urine