Human TGF beta 1 ELISA Kit
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  • Human TGF beta 1 ELISA Kit

Human TGF beta 1 ELISA Kit

Catalog # FM-E100128

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$649.00
Quantity

Assay Range

15.6 - 1,000 pg/mL  

Sensitivity

10.5 pg/mL

Size

96T

Storage

Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC

Assay Principle

Sandwich ELISA

Sample Volume

100 µL final volume, dilution factor varies on samples. Human serum dilution betweeen 1:40 to 1:100.

Detection Method

Colorimetric

 

Kit Components

 1. Recombinant  Human TGF beta1 standard: 2 vials

 2. One 96-well plate coated with anti- Human TGF beta1  Ab

 3. Sample diluent buffer: 12 mL - 2

 4. Detection antibody: 60 µL, dilution 1:180

 5. Streptavidin-HRP: 300 µL, dilution 1:40

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. Chromogen-A, 6 mL; Chromogen-B, 6 mL.

 9. Stop solution: 10 mL x1

10. Washing solution (20x): 25 mL x1.


 

Precision          
             
  Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
CV (%) 4.3 4.7 3.9 6.3 8.5 9.6
Average CV (%) 4.3 8.1
             
Recovery          
             
Standard Added Value 20- 800 pg/ml
Recovery % 93 - 105%
Average Recovery % 99%
             
Linearity          
             
Average Percentage of Expected Value (%)
Sample Dilution Plasma Serum
2 x  102% 96%
4 x  105% 99%
8 x 103% 98%



 product citations

1. The Potential effect of G915C polymorphism in regulating TGF-β1 transport into Endoplasmic Reticulum for cytokine production

Susianti H, Gunawan A, Putri JF, Purnomo BB, Handono K, Kalim H

 Bioinformation. 2014 Aug 30;10(8):487-90

 Sample: Urine

2. Analysis of urinary TGF-β1, MCP-1, NGAL, and IL-17 as biomarkers for lupus nephritis

 Susianti H, Iriane VM, Dharmanata S, Handono K, Widijanti A, Gunawan A, Kalim H

 Pathophysiology. 2015 Mar;22(1):65-71

Sample: Urine


Data sheet

Background
Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation,
Assay principle
This Human TGF beta 1 ELISA Kit is based on standard sandwich enzyme-linked immunosorbent
assay technology. Human TGF beta 1 specific antibody has been precoated onto 96-well plate.
The test samples and the biotinylated Human TGF beta 1 specific detecti
Alternate Names
DPD1; latency-associated peptide; TGFB; TGFB1; TGF-beta 1 protein; TGFbeta 1; TGF-beta 1; TGFbeta; TGF-beta-1; transforming growth factor beta-1
Gene ID
7040
UniProt ID
P01137
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