Human sL-Selectin ELISA Kit

Kit Components

 1. Recombinant  Human sL-Selectin standard: 2 vials

 2. One 96-well plate precoated with anti-Human sL-Selectin  Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1.

10. Washing solution (20x): 25 mL x1.

Background

Human L-Selectin, also known as leukocyte selectin, Leukocyte adhesion molecule 1 (LAM-1), Leukocyte surface antigen Leu-8, Leukocyte-endothelial cell adhesion molecule 1 (LECAM1) CD62L, LAM-1, LECCAM-1, TQ1, Leu-8, DREG, lymph node homing receptor, MEL-14 antigen, is a cell surface glycoprotein belonging to the selectin family. L-Selectin is characterized by an NH2-terminal calcium-dependent lectin domain, an EGF-like domain, two CRP-like domains, a transmembrane sequence and a short cytoplasmic domain. Human and mouse L-Selectin show the same domain structure and are 77% amino acid sequence identical.

L-selectin is an adhesion/homing receptor that plays important roles in lymphocyte-endothelial cell interactions. Three ligands (GlyCAM-1, sgp90/CD34, MAdCAM-1) for L-Selectin have been identified on endothelial cells, all containing O-glycosylated mucin or mucin-like domains. Two forms of soluble L-Selectin (sL-Selectin) with different molecular weight have been identified. The lymphocyte form is about 62 kDa, while the fragment derived from neutrophils is 75-100 kDa. sL-Selectin retains bioactivity, and at high concentrations can inhibit binding of lymphocytes to endothelium.