Canine IL-2 ELISA Kit

Catalog # NR-E10015

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$625.00
Quantity

Assay Range

7.81-500 pg/mL

Sensitivity

1.0 pg/mL

Specificity

No cross-reaction with other related substances detected

Size

96T

Storage

Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC

Assay Principle

Sandwich ELISA

Sample Volume

100 µL final volume, dilution factor varies on samples

Sample Type

Cell culture supernatants, serum, plasma, or other suitable sample solution.

Detection Method

Chromogenic

 

 

Kit Components

 

 1. Recombinant Canine IL-2 standard: 2 vials

 2. One 96-well plate coated with Canine IL-2  Ab

 3. Diluent buffer 1: 12 mL - 1

 4. Detection antibody: 1 vial

 5.Streptavidin-HRP: 1 vial

 6. Diluent buffer 2: 12 mL x1  

 7. Diluent buffer 3: 12 mL x1

 8. Chromogenic solution  A:  6 mlx1

 9. Chromogenic solution  B: 6 mlx1

10. Stop solution:  6 mL x1

11. Washing solution (20x):  25 mL x1

 

Background     

Interleukin 2 (IL-2), also known as T cell growth factor (TCGF), is a member of the common gamma chain (γc) cytokine family. The 153 amino acid (aa) human IL-2 precursor contains a 20 aa signal sequence and a 133 aa mature region with one O-linked glycosylation site at Thr3, plus three cysteines, two of which form an intrachain disulfide bond that is essential for IL-2 bioactivity. Human IL-2 shares approximately 60% sequence identity with mouse IL-2, and is functional on mouse IL-2 responsive cells. IL-2 is expressed and secreted on γδT cells, activated conventional CD4+ and CD8+ T cells, neurons, microglia, and hematopoietic stem cells in humans.

 

IL-2 first binds to IL-2 receptorα (IL-2Rα)/ CD25 in the binary receptor complex then recruits IL-2Rβ/CD122 and IL-2Rγ/CD132 to trigger a variety of biological processes. IL-2 plays a key role in the development, maintenance and function of regulatory T cells and in promoting the development of activated CD8+ T cells into memory cells.

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