Data sheet
| Background | NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor‑dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system. |
More info
Product name: Dog/Canine Nerve Growth Factor NGF ELISA Kit
Catalog No. BG-CAN11645
Size: 96T
Range: 31.2-2000 pg/mL
Sensitivity: Less than 10 pg/mL
Storage: Store at 2-8 °C.
Expiration: Six months.
Application: cell culture, supernatant, serum, plasma and other suitable sample solution.
Kit Components:
| 1.Canine Beta-NGF standard: | 10 ng/vial x 2 |
| 2. 96-well plate pre-coated with anti-Canine Beta-NGF Ab: | 1 |
| 3. Sample Diluent buffer : | 12 ml × 2 |
| 4. Detection antibody: | 1x180 μl , dilution 1:60 |
| 5. Streptavidin-HRP: | 1x300 μl, dilution 1:40 |
| 6. Antibody Diluent buffer: | 12 ml |
| 7. Streptavidin HRP diluent buffer: | 12 ml |
| 8. Chromogen Solution A: | 6 ml |
| 9. Chromogen Solution B: | 6 ml |
| 10.Stop Solution: | 6 ml |
| 11.20 × Wash Buffer: | 25 ml |
| 12.Plate sealers | 2 |
| 13.Package insert | 1 |
Introduction
Nerve growth factor (NGF) is a small secreted protein that is important for the growth, maintenance, and survival of certain target neurons. It also functions as a signaling molecule. It is perhaps the prototypical growth factor, in that it is one of the first to be described. NGF is critical for the survival and maintenance of sympathetic and sensory neurons. Without it, these neurons undergo apoptosis.
Dog/Canine Nerve Growth Factor (Ngf) ELISA Kit
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Different lot may vary, please follow the instruction coming with the kit.