DNA Topoisomerase-I Human Recombinant ( TOP1 Human )

Data sheet

Formulation

TOP1 is supplied in 16mM HEPES buffer pH-7.5, 400mM sodium chloride, and 20% glycerol.

Purity

Greater than 95% as determined by SDS-PAGE.

Description

DNA Topoisomerase-I Human Recombinant produced in SF9 is a glycosylated, polypeptide chain having a molecular mass of 91,576 Dalton (excluding glycosilation) 102 kDa total mass. The TOP1 is expressed with a -6xHis tag and purified by proprietary chromatog

Protein Background

DNA toposisomerase I is a key nuclear enzyme that interconverts supercoiled DNA to the required topological conformations for normal DNA replication and transcription. This enzyme is the target antigen for the so-called Scl-70 autoantibodies. Scl-70 antib

Expression host

Sf9 insect cells.

Synonyms

DNA topoisomerase 1, EC 5.99.1.2, DNA topoisomerase I, TOP1, Scl-70.

Reagent Appearance

Sterile Filtered clear solution.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles.