Thermostable dUTPase Pyrococcus Fruriosus Recombinant ( DUT Pyrococcus Fruriosus )

Data sheet

Formulation

dUTPase is supplied in 20mM Tris-HCl (pH 8.2), 1mM DTT, 0.1mM EDTA, 100mM KCl, 0.1% Nonidet P40, 0.1% Tween 20 and 50% glycerol at a concentration of 10U/ul of the enzyme.

Purity

Greater than 97.0% as determined by SDS-PAGE.

Description

Thermostable dUTPase (pyrococcus fruriosus) maximizes the efficiency of high-fidelity PCR (using proofreading DNA polymerases). It removes contaminating dUTP present in PCR reactions and dNTP solutions. The presence of dUTPase in a proofreading DNA polyme

Expression host

Escherichia Coli.

Synonyms

Thermostable dUTPase, dUTPase.

Reagent Appearance

Sterile filtered liquid formulation.

Stability

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Specific Activity

10u/ug.

Activity

(A) Measured by its ability to hydrolyze dUTP to dUMP in reaction buffer 20mM Hepes pH7.5, 150mM KCI, 5mM MgCl2, 10mM dUTP at 85 Centigrade for 1 hour. The PPi production was quantified by using the enzymatic determination kit from Sigma.(B) Enhancing PCR

Unit Defenition

One unit of enzyme catalyzes hadrylazation of 10 nanomoles of dUTP to dUMP in one hour at 85 Centigrade.