RNA ( guanine-7-) Methyltransferase Human Recombinant ( RNMT Human )

Data sheet

Formulation

RNMT solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH 8.0) 2mM DTT, 20% glycerol and 100mM NaCl.

Purity

Greater than 90.0% as determined by SDS-PAGE.

Description

RNMT produced in E.Coli is a single, non-glycosylated polypeptide chain containing 496 amino acids (1-476 a.a.) and having a molecular mass of 57kDa.RNMT is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic technique

Protein Background

RNMT is a widely expressed nuclear protein which is a member of the mRNA cap methyltransferase family. Cap-dependent mRNA translation requires the methylation of the mRNA guanosine cap by RNMT. RNMT catalyzes the transfer of a methyl group from AdoMet (S-

Expression host

Escherichia Coli.

Synonyms

mRNA cap guanine-N7 methyltransferase, RG7MT1, mRNA (guanine-N(7)-)-methyltransferase, mRNA cap methyltransferase, hCMT1, hMet, hcm1p, RNMT, KIAA0398, MET, RG7MT1, hCMT1c, DKFZp686H1252.

Reagent Appearance

Sterile filtered colorless solution.

Stability

RNMT Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MANSAKAEEY EKMSLEQAKA SVNSETESSF NINENTTASG TGLSEKTSVC RQVDIARKRK EFEDDLVKES SSCGKDTPSK KRKLDPEIVP EEKDCGDAEG NSKKRKRETE DVPKDKSSTG DGTQNKRKIA LEDVPEKQKN LEEGHSSTVA AHYNELQEVG LEKRSQSRIF YLRNFNNWMK SVLIGEFLEK VRQKKKRDIT VLDLGCGKGG DL