T4 DNA Ligase Recombinant ( T4 DNA )

Catalog # PT_42181

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$218.00
DescriptionT4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' -phosphate and 3' -hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded
Product Size: 20,000IU
Quantity

Data sheet

Formulation
50mM Tris-HCl (pH 7.8 at 25°C), 10mM MgCl2, 10mM DTT, 1mM ATP, 25 ug/ml BSA and DNA (0.1 to 1 um in 5 termini). Optimal ligation occurs at 16C.
Applications
Cloning of restriction fragments.Joining linkers and adapters to blunt-ended DNA.
Description
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between juxtaposed 5' -phosphate and 3' -hydroxyl termini in duplex DNA or RNA. This enzyme will join blunt end and cohesive end termini as well as repair single stranded nicks in duplex DNA,
Expression host
Escherichia Colilambda lysogen NM 989.
Synonyms
DNA ligase 4, EC 6.5.1.1, DNA ligase IV, Polydeoxyribonucleotide synthase [ATP] 4.
Reagent Appearance
Sterile filtered liquid formulation having a concentration of 167,000 U/ml.
Biological Activity
One Weiss unit is equivalent to circa 67 cohesive-end ligation units. T4 DNA Ligase is strongly inhibited by NaCl or KCl if the concentration is > 200mM. Ligation of blunt-ended and single-base pair overhang fragments requires about 50 times as much enzym
Unit Defenition
1. One unit is defined as the amount of enzyme required to give 50% ligation of Hind III fragments of DNA (5 DNA termini concentration of 0.12 uM, 300- ug/ml) in a total reaction volume of 20 ul in 30 minutes at 16°C in 1X T4 DNA Ligase Reaction Buffer.
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