Bone Morphogenetic Protein-2 Human Recombinant ( BMP 2 Human )

Catalog # PT_40030

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$201.00
DescriptionBone Morphogenetic Protein-2 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated, Polypeptide chain containing 115 amino acids and having a molecular mass of 26018 Dalton. The BMP-2 is purified by proprietary
Product Size: 2ug
Quantity

Data sheet

Formulation
BMP2 was lyophilized from a concentrated (1mg/ml) sterile solution containing 10mM sodium citrate pH=3.5.
Solubility
It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-2 in sterile 20mM AcOH (acetic Acid) not less than 100ug/ml, which can then be further diluted to other aqueous solutions.
Purity
Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.
Description
Bone Morphogenetic Protein-2 Human Recombinant produced in E.Coli is a homodimeric, non-glycosylated polypeptide chain containing 2x115 amino acids and having a molecular mass of 26kDa. The BMP-2 is purified by proprietary chromatographic techniques.
Protein Background
BMP2 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone morphogenic protein induces bone formation. BMP2 is a candidate gene for the autosomal dominant disease of fibrodysplasia (myositis) ossificans progressiva.
Expression host
Escherichia Coli.
Synonyms
BMP-2, BMP2A.
Reagent Appearance
Sterile Filtered White lyophilized (freeze-dried) powder.
Stability
Lyophilized Bone Morphogenetic Protein-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP2 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is
Amino acid sequence
MQAKHKQRKR LKSSCKRHPL YVDFSDVGWN DWIVAPPGYH AFYCHGECPF PLADHLNSTN HAIVQTLVNS VNSKIPKACC VPTELSAISM LYLDENEKVV LKNYQDMVVE GCGCR.
Biological Activity
The ED50 as determined by its ability to induce alkaline phosphatase production by ATDC-5 cells is 0.5-1.0 ug/ml.
References
Title:β-Catenin Signaling Pathway Is Crucial for Bone Morphogenetic Protein 2 to Induce New Bone Formation.Publication:First Published on November 3, 2006, doi: 10.1074/jbc.M602700200 January 5, 2007 The Journal of Biological Chemistry, 282, 526-533.
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