Bone Morphogenetic Protein-7 Human Recombinant, CHO ( BMP 7 Human, CHO )

Data sheet

Formulation

BMP-7 was lyophilized from a concentrated (1mg/ml) sterile solution containing 1% sucrose, 1.2% mannitol, 20mM glycine and 0.05% polysorbate 20 pH-4.

Solubility

It is recommended to reconstitute the lyophilized Bone Morphogenetic Protein-7 in 0.5mg/1ml sterile/ endotoxin free water.

Purity

Greater than 97.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Description

N-TERMINAL---Human BMP-2 (Met 1 Arg 282) Human BMP-7 (Ser 293 Arg 431)---C-TERMINAL. The DNA sequence encoding the human BMP-2 signal peptide and propeptide (1~282 amino acid) fused to the human rhBMP-7 mature chain (293~431 amino acid) was expressed in

Protein Background

The bone morphogenetic proteins (BMPs) are a family of secreted signaling molecules that can induce ectopic bone growth. Many BMPs are part of the transforming growth factor-beta (TGFB) superfamily. BMPs were originally identified by an ability of deminer

Expression host

Chinese Hamster Ovarian Cells.

Synonyms

Osteogenic Protein 1, BMP-7.

Stability

Lyophilized BMP-7 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP 7 Human should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to

Biological Activity

Measured in alkaline phosphatase activity assay using MC3T3-E1 cells. The ED50 for this effect is

References

1.Title:Bone morphogenetic protein 7 induces mesenchymal-to-epithelial transition in melanoma cells, leading to inhibition of metastasis.Publication:Article first published online: 30 JUL 2009 DOI:10.1111/j.1349-7006.2009.01301.x © 2009 Japanese Canc