Bone Morphogenetic protein-4 Human Recombinant, Active ( BMP 4 Human, Active )

Data sheet

Formulation

The BMP-4 was lyophilized from 0.7mg/ml in 2xPBS+6% ethanol.

Solubility

It is recommended to reconstitute the lyophilized BMP-4 in sterile 4mM HCl containing 0.1% endotoxin-free recombinant HSA.

Purity

Greater than 95% as obsereved by SDS-PAGE.

Description

BMP-4 Human Recombinant produced in HEK cells is a glycosylated disulfide linked homodimer, having a total molecular weight of 34kDa.The BMP-4 is purified by proprietary chromatographic techniques.

Protein Background

The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. The superfamily includes large families of growth and differentiation factors. Bone morphogenetic prote

Expression host

HEK.

Synonyms

BMP4, ZYME, BMP2B, BMP2B1.

Stability

Lyophilized BMP4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution BMP-4 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a c

Amino acid sequence

GASHASLIPE TGKKKVAEIQ GHAGGRRSGQ SHELLRDFEATLLQMFGLRR RPQPSKSAVI PDYMRDLYRL QSGEEEEEQI HSTGLEYPER PASRANTVRS FHHEEHLENI PGTSENSAFR FLFNLSSIPE NEAISSAELR LFREQVDQGP DWERGFHRIN IYEVMKPPAE VVPGHLITRL LDTRLVHHNV TRWETFDVSP AVLRWTREKQ PNYGLAIEVT HLHQTRTHQG QHV

Physical Appearence

Sterile Filtered White lyophilized (freeze-dried) powder.

Biological Activity

The specific activity was determined by the dose dependent induction of alkaline phosphatase production in the ATDC-5 cell line (Mouse chondrogenic cell line) and is typically 4.53ng/ml corresponding to a Specific Activity of 220,750.55IU/mg.