Guanosine Monophosphate Reductase 2 Human Recombinant ( GMPR2 Human )

Data sheet

Reconstitution

1mg/ml in PBS (after reconstitution).

Formulation

GMPR2 1mg/ml solution contains 20mM Tris pH-8, 1mM DTT and 10% glycerol.

Purity

Greater than 90% as determined by SDS-PAGE.

Description

GMPR2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 368 amino acids (1-348 a.a.) and having a molecular mass of 40 kDa. GMPR2 is fused to a 20 amino acid His-tag at N-terminus and purified by proprietary

Protein Background

GMPR2 is the single known metabolic step by which guanine nucleotides can be transformed to the pivotal precursor of both adenine and guanine nucleotides. GMPR2 catalyzes the permanent NADPH-dependent reductive deamination of GMP to IMP, and is involved i

Expression host

Escherichia Coli.

Synonyms

GMP reductase 2, Guanosine 5''-monophosphate oxidoreductase 2.

Stability

GMPR2 Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles.

Amino acid sequence

MGSSHHHHHH SSGLVPRGSH MPHIDNDVKL DFKDVLLRPK RSTLKSRSEV DLTRSFSFRN SKQTYSGVPI IAANMDTVGT FEMAKVLCKFSLFTAVHKHY SLVQWQEFAG QNPDCLEHLA ASSGTGSSDF EQLEQILEAI PQVKYICLDV ANGYSEHFVE FVKDVRKRFP QHTIMAGNVV TGEMVEELIL SGADIIKVGI GPGSVCTTRK KTGVGYPQLS AVMECADAAH GLK

Physical Appearence

Sterile filtered colorless solution.

Antibody Properties

Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use.

Titer

Antibody is shipped lyophilized at ambient temperature.

Protein content

By direct ELISA (against r.hepatitis vaccine), 1:10,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories).

Application And Suggested Dilutions

This antibody recognizes both the AD and the AY antigens in direct ELISA and works in capture ELISA with anti-hepatitis Ag-2.