Cardiac Actin ( c-Actin )

Data sheet

Formulation

The protein was lyophilized from a 1mg/ml solution containing 10mM Tris-acetate buffer pH 8.0, 0.2mM CaCl2, 0.2mM ATP, 1mM DTT and 0.5% SDS.

Solubility

It is recommended to reconstitute the lyophilized Cardiac Actin in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE.

Applications

Protein standard in 1D and 2D SDS gelelectrophoresisImmunoassays Immunization.

Description

Ultra pure Cardiac Actin having a Molecular mass of 43,000 dalton.

Protein Background

Alpha-cardiac actin belongs to the actin family which is comprised of three main groups of actin isoforms, alpha, beta, and gamma. The alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Defects in the cardia

Expression host

Bovine Heart.

Synonyms

Actin alpha cardiac muscle 1, Alpha-cardiac actin, ACTC1, ACTC.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Cardiac-Actin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Cardiac Actin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recom