Actin ( Actin )

Data sheet

Formulation

The protein was lyophilized from a 1mg/ml solution containing 10mM Tris/HCl buffer pH 8.0, 0.2mM CaCl2, 0.2mM ATP, 1mM DTT and 0.5% (w/v) SDS.

Solubility

It is recommended to reconstitute the lyophilized Actin in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions.

Purity

Greater than 98.0% as determined by SDS-PAGE.

Description

Ultra pure Actin consists in the alpha-skeletal muscle isoform and is purified from rabbit striated muscle.The purification method used (according to Spudich & Watts) results in a highly purified protein having a Molecular mass of 43,000 dalton.

Protein Background

Actin is a muscle protein localized in the I band of the myofibrils; acting along with myosin, it is responsible for contraction and relaxation of muscle. Each actin protomer binds one molecule of ATP and has one high affinity site for either calcium or m

Expression host

Rabbit Muscle.

Reagent Appearance

Sterile Filtered White lyophilized (freeze-dried) powder.

Stability

Lyophilized Actin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Actin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a