Adiponectin Human Recombinant, HEK ( Acrp30 Human, HEK )

Data sheet

Formulation

Filtered (0.4um) and lyophilized from 0.5mg/ml in 50mM phosphate Buffer, 75mM NaCl, pH 7.4.

Solubility

Add deionized water to prepare a working stock solution of approximately 0.5mg/ml and let the lyophilized pellet dissolve completely. Product not sterile! Please filter the product by an appropriate sterile filter before using it in cell culture.

Purity

Greater than 98% as determined by HPLC and SDS PAGE.

Description

The Acrp30 Human Recombinant produced in HEK293 cells is fused with FLAG tag.

Protein Background

Adiponectin is a recently discovered 244 amino acid protein, the product of the apM1 gene, which is physiologically active and specifically and highly expressed in adipose cells (Adipokine). The protein belongs to the soluble defense collagen super family

Expression host

HEK293 (Human Embryonic Kidney cell line).

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Reagent Appearance

White lyophilized (freeze-dried) powder.

Stability

For long term, store lyophilized AdipoQ at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4

Amino acid sequence

ETTTQGPGVL LPLPKGACTG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHIVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTNDYKDDDDK