Adiponectin Human Recombinant, Trimeric form ( Acrp30 Human, Trimeric )

Data sheet

Formulation

Filtered (0.4um) and lyophilized from 0.5mg/ml in 0.05M phosphate buffer, 0.075M NaCl, pH 7.4.

Solubility

Add deionized water to a working volume of 0.5mg/ml and let the lyophilized pellet dissolve completely. Product is not sterile! Please filter the product by an appropriate sterile filter before using it in the cell culture.

Purity

Greater than 95% as determined by SDS-PAGE.

Description

Trimeric form of Adiponectin Human trimeric form was expressed in HEK293 cells. The cysteine 39 was replaced with Alanine (C39A) 9. hAd-C39A can only form a trimer, but not a hexamer or an HMW form.

Protein Background

Adiponectin is a hormone exclusively expressed from adipose tissue.Many studies demonstrate that Adiponectin has direct anti-diabetic, anti-atherogenic and anti-inflammatory functions. APM-1 can increase insulin sensitivity of skeletal muscle. Attenuate h

Expression host

HEK293 (Human embryonic kidney cell line).

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Reagent Appearance

Filtered White lyophilized (freeze-dried) powder.

Stability

Store lyophilized Adiponectin at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino acid sequence

ETTTQGPGVL LPLPKGAATG WMAGIPGHPG HNGAPGRDGR DGTPGEKGEK GDPGLIGPKG DIGETGVPGA EGPRGFPGIQ GRKGEPGEGA YVYRSAFSVG LETYVTIPNM PIRFTKIFYN QQNHYDGSTG KFHCNIPGLY YFAYHIVYMK DVKVSLFKKD KAMLFTYDQY QENNVDQASG SVLLHLEVGD QVWLQVYGEG ERNGLYADND NDSTFTGFLL YHDTNDYKDD DD

Activity

ED50= 3-8.5 g/ml, as determined by its ability to inhibit proliferation of HASMCs induced by HB EGF.