Adiponectin Mouse Recombinant, HEK ( Acrp30 Mouse, HEK )

Data sheet

Formulation

Filtered (0.4um) and lyophilized from 0.5 mg/ml in PBS buffer.

Solubility

It is recommended to reconstitute the lyophilized Acrp30 Mouse in sterile 18M-cm H2O at 0.5mg/ml, which can then be further diluted to other aqueous solutions.

Purity

Purity of adiponectin mouse recombinant is >98% (HPLC and SDS PAGE analyzed).

Description

The Acrp30 Mouse Recombinant is fused with FLAG tag having a total Mw of 26kDa.

Protein Background

Adiponectin is an important negative regulator in hematopoiesis and immune systems. It may be involved in ending inflammatory responses through its inhibitory functions. Inhibits endothelial nf-kappa-b signaling through a camp-dependent pathway. Inhibits

Expression host

HEK293 (Human embryonic kidney cell line).

Synonyms

Acrp30, AdipoQ, GBP-28, APM-1, ACDC.

Stability

Store lyophilized APM-1 at -20°C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4°C for a limited period of time; it does not show any change after two weeks at 4°C.

Amino acid sequence

EDDVTTTEEL APALVPPPKG TCAGWMAGIP GHPGHNGTPG RDGRDGTPGE KGEKGDAGLL GPKGETGDVG MTGAEGPRGF PGTPGRKGEP GEAAYMYRSA FSVGLETRVT VPNVPIRFTK IFYNQQNHYD GSTGKFYCNI PGLYYFSYHI TVYMKDVKVS LFKKDKAVLF TYDQYQEKNV DQASGSVLLH LEVGDQVWLQ VYGDGDHNGL YADNVNDSTF TGFLLYHDTN DY

Biological Activity

Full-length mouse adiponectin activates AMP-activated protein kinase in hepatocyte and activates AMPK in HepG2 human hepatocytes at a concentration of 1000ng/ml corresponding to a Specific Activity of 1000IU/mg. Mouse adiponectin mammalian cell derived in