Avidin ( Avidin )

Data sheet

Solubility

It is recommended to reconstitute the lyophilized Avidin in sterile 18M-cm H2O not less than 100ug/ml or more than 10mg/ml solutions.

Purity

Greater than 92.0%.

Inactivation

BDNF quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 1.6 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of pr

Description

Avidin is a glycosylated polypeptide chain having a molecular mass of 68kDa and containing 4 subunits each with a binding site for biotin. The Avidin is purified by affinity chromatographic techniques.The purification procedure ensures minimal contaminati

Protein Background

Avidin is a tetrameric protein of 4 identical subunits (homotetramer) each of which can bind to biotin with a high degree of affinity and specificity. Avidin molecular weight in its tetrameric form is estimated to be between 66-69 kDa. Avidin is produced

Expression host

Hen's egg white.

Synonyms

Avidin, AVD, AVID.

Reagent Appearance

Sterile Filtered white lyophilized powder.

Stability

Lyophilized Avidin although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution Avidin should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add

Activity

16 units/mg protein, 1 unit binds 1ug biotin.